LOCUS       40924_4694        5878 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cyanobacterial shuttle vector pAQJ4-MCS DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Onizuka T, Endo S, Akiyama H, Kanai S, Hirano M, Yokota A, Tanaka S,
            Miyasaka H.
  TITLE     The rbcX gene product promotes the production and assembly of 
            ribulose-1,5-bisphosphate carboxylase/oxygenase of Synechococcus sp.
            PCC7002 in Escherichia coli
  JOURNAL   Plant Cell Physiol. 45 (10), 1390-1395 (2004)
  PUBMED    15564522
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Miyasaka H, Tanaka S, Onizuka T, Akiyama H.
  TITLE     Cyanobacterial shuttle vector pAQJ4-MCS
  JOURNAL   Published Only in Database (2009)
REFERENCE   3  (bases 1 to 5878)
  AUTHORS   Miyasaka H, Tanaka S, Onizuka T, Akiyama H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2009) Contact:Takuo Onizuka Toray Research Center,
            Inc., Biological Science Laboratories; 1111 Tebiro, Kamakura, 
            Kanagawa 248-0036, Japan
REFERENCE   4  (bases 1 to 5878)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol."; date: "2004"; volume: "45"; issue: "10"; pages: 
            "1390-1395"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2009)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (04-FEB-2009) Contact:Takuo Onizuka Toray Research Center, Inc., 
            Biological Science Laboratories; 1111 Tebiro, Kamakura, Kanagawa 
            248-0036, Japan"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    401..406
                     /label=XbaI cloning site
                     /note="XbaI cloning site"
     misc_feature    407..412
                     /label=PstI cloning site
                     /note="PstI cloning site"
     misc_feature    413..418
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     misc_feature    417..422
                     /label=SmaI cloning site
                     /note="SmaI cloning site"
     misc_feature    421..426
                     /label=KpnI cloning site
                     /note="KpnI cloning site"
     misc_feature    427..432
                     /label=ClaI cloning site
                     /note="ClaI cloning site"
     misc_feature    433..438
                     /label=EciRI cloning site
                     /note="EciRI cloning site"
     CDS             complement(625..3456)
                     /codon_start=1
                     /gene="orf943"
                     /product="ORF943"
                     /function="essential for the replication of the plasmid in 
                     cyanobacteria"
                     /label=orf943
                     /protein_id="BAH22361.1"
                     /translation="MTPYEYLLYSDEPALRRNDGRLRDTWLKRYAFVEHGGWWCSGIDI
                     KTGKDSLWGCFKGDRPRKDREDKKPIKYEHPPRVATEIFTLKVDRGTWRKIAKRHKVEL
                     PETDQGFWEWVLAHPELPIIITEGAKKAGALLTAGYCAIGLPGIYNGYRTPKNDHGEPM
                     RQLRHLIPELDLLAKNNRAIAFCFDQDKKPKTIKAVNGAIQTTGALLEKAGAKVSVITW
                     HQDAKGVDDLIVEHGAKALHNRYKHRKPLAVWEMDNLTDITTQVDLTVDQRYLDIDPRA
                     IPKDAQIIFIKSAKGTGKTEWLGKIVKLAQDDCARVLVLTHRIQLAKELARRLDIDHIS
                     ELDSSPTGGALGMAMCIDSLHPDSQAHFNFMEWHGAHIVLDEIEQVLGHALGSSTCTQD
                     RAKILETFYNLILYALRTGGKLYCSDADLSPISYELIKYILDGCEFKPFTILNTYKPCL
                     EQQRDLFFYEGNDPRDLLTNLRQAIENGEKTLVFTAAQKTASTYSTQNLESLFREKYPD
                     KRILRIDAESVAEPGHPAYGCIDSLNAILPLYDIVLCSPAVETGVSIDIKDHFDSVWGM
                     GSGVQTVNGFCQGLERLRDNVPRHVWIPKFSPHSNRIANGGYTAKAIARDQHRYAELTH
                     KLIGEHAAECSGLEDSLKPFLWAYCRYAALANRGFGSYREAILNKLLSEGYVQKDLSEI
                     DPALAKDYRDELKAVKDHNYLQERVAISKVENPDDRQYEKLKRQRAKSETERHQERHGK
                     LSRSYGLTVTPELVEKDDDGWYSQLQLEYYLTVGKAFCSARDRAKYDQLQHEGFVFKPD
                     INRRSLSPKIHLLELLNIHQFLKPGVTFTGASLEGFKENCLRYAKPIKWILGRTITDKM
                     SPLEIAQALLGKLDRKLEYKGRFGSRDNRQRVYEAIAPNDQREKVFAHWLQRDQAKLGA
                     VSNPCINRFIQEA"
     gene            complement(625..3456)
                     /gene="orf943"
                     /label=orf943
     primer_bind     complement(3657..3673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3681..3697)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3705..3735)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3750..3771)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4059..4647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4821..5678)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5679..5783)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt tctagactgc agggatcccg
      421 ggtaccatcg atgaattcga gctcatgccc ccggtagtgc cctcaccctc agtgaggctt
      481 atgatctggc acagtctagg ggctaccaga agagcaaagg cgctttccgc aagggcataa
      541 aacgtcccga taccgcagag caaatcttga gattgtttgg gattgccaag ggagagctgc
      601 acaacgagta tttcgatcac gggtctaagc ctcctgaata aatctattta tacaggggtt
      661 ggacacggcc cctaattttg cttggtcacg ctgtaaccaa tgagcaaaga ccttttcgcg
      721 ctgatcgtta ggggcgatcg cctcatagac ccgctgacgg ttatcccgcg atccaaagcg
      781 ccccttgtat tccaatttcc ggtcaagctt gcctaggagc gcctgagcaa tttcgagcgg
      841 gctcattttg tcggtgatcg ttctgccaag aatccacttg atcggcttgg cataccgcaa
      901 acaattttcc ttgaaccctt caaggctcgc cccggtgaat gtcacccctg gttttaagaa
      961 ctgatggatg ttgagtagct ctaacaggtg aatctttggt gagagcgatc gcctgttgat
     1021 atccggctta aatacaaagc cctcatgttg gagctggtca tatttcgccc ggtcgcgggc
     1081 agagcagaat gctttcccaa cggttaagta gtattcgagc tggagctgag agtaccaccc
     1141 atcatcatct ttctcgacaa gctcaggggt cacagttaac ccataggagc gagaaagttt
     1201 cccgtggcgt tcttggtgcc gttccgtctc agatttcgcc cgctgacgct tcagtttttc
     1261 gtactggcga tcgtcaggat tttctacttt agaaatcgca accctttcct gtagataatt
     1321 atggtctttc accgctttta attcgtctcg ataatcctta gccaatgctg gatcgatttc
     1381 gctcaaatct ttctgtacat agccctcaga aagcagctta tttaaaatcg cttcccggta
     1441 actgccaaag ccacggttag caagcgccgc atagcgacaa taggcccaaa ggaatggttt
     1501 taaagaatct tctaacccac tgcattcagc ggcgtgctca ccgattaatt tgtgggtgag
     1561 ctctgcatag cggtgctggt cacgggcgat cgccttagcg gtgtagcccc cattggcgat
     1621 ccggttcgag tgtggggaaa atttcggtat ccaaacatgg cgagggacgt tatcccgtaa
     1681 ccgctctagc ccttggcaga aaccgttaac ggtctgaacc cctgaaccca tgccccaaac
     1741 agaatcaaaa tgatccttga tatcgatact caccccggtc tcgacagcgg gcgagcagag
     1801 cacaatatcg tacaggggca aaattgcatt cagcgagtca atacaaccat aggcagggtg
     1861 ccccggttca gcgaccgatt cagcatcgat tctcaagatt ctcttatcgg gatatttctc
     1921 cctaaagagt gattctaagt tctgcgtgct gtaggtcgat gcggtctttt gggcagcggt
     1981 aaagacaagt gttttctcac cgttctcaat cgcttgtctg agattggtta acaggtctct
     2041 agggtcattc ccttcataga aaaacaggtc tctttgttgc tctaaacagg gcttataggt
     2101 attcagaatg gtgaatggtt tgaactcaca accgtcaaga atgtacttga ttagctcata
     2161 ggagatggga gataaatcag catcagagca gtagagtttt ccgcctgtcc ttagggcata
     2221 aaggattagg ttgtagaacg tttcaaggat tttcgcccgg tcttgggtac aggtcgagct
     2281 acccaaagcg tgccctaaaa cttgctcgat ttcgtctagg acaatgtgag cgccgtgcca
     2341 ttccatgaaa ttaaaatgag cttggctatc gggatgtagg ctatcgatac acatcgccat
     2401 ccctagagcg cccccggtcg ggctactgtc gagctcgcta atgtgatcga tatcgagacg
     2461 gcgggcgagc tccttggcta attggatgcg gtgagtcaaa accagtacgc gagcgcaatc
     2521 atcttgggcg agcttaacga ttttccctaa ccattctgtt ttcccggtgc ccttggcaga
     2581 tttaatgaaa ataatctgag catccttggg gatagcacgc ggatcgatgt cgagatagcg
     2641 ctgatcgacc gttagatcga cttgcgtggt gatatcggtg agattatcca tctcccagac
     2701 tgctaagggc ttgcggtgct tgtagcggtt atggagtgct ttcgctccgt gctcgacgat
     2761 cagatcatca acacctttcg cgtcctggtg ccaggtgatc accgatactt tcgccccggc
     2821 cttctctagg agtgccccgg tagtttggat cgccccgttc actgccttga tcgtcttggg
     2881 tttcttgtct tggtcaaaac agaaggcgat cgcccggtta tttttcgcca acaggtcaag
     2941 ctctggaatg aggtgccgta gctgtcgcat tggctcgcca tggtcatttt ttggcgttct
     3001 gtagccgttg taaatccccg gtagaccgat ggcgcaataa ccagcggtta ggagggcccc
     3061 tgccttcttc gcgccctcag tgatgatgat cggtagctca ggatgggcta gtacccattc
     3121 ccagaagcct tgatcggttt ctggtagctc gactttgtgg cgcttggcaa tcttgcgcca
     3181 ggtgccccgg tctaccttga gagtgaaaat ttcggtggct acccgtggcg ggtgctcata
     3241 cttgatcggt ttcttatctt cgcgatcttt acgggggcga tcgcctttga agcaacccca
     3301 aagagaatct ttcccggtct taatatcgat gcctgagcac caccaaccgc catgctcgac
     3361 aaaggcatat cgcttaagcc aagtatcccg aagcctgcca tcattgcggc gtaatgctgg
     3421 ctcatcactg tagagcaggt attcataggg cgtcatccct gacaacgatc taacatttaa
     3481 ccgggcaatc tcaggggcga ccccggaacc ctctacccat tccctatagt gagattctgc
     3541 gataaaatga gaacggttct cactgacgct actcgctaaa tctgccccgg tattactgct
     3601 agatgtaaaa ttatctgata cgatcacagt gagttaagga gcttggcgta atcatggtca
     3661 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3721 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3781 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3841 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3961 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4021 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4081 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4141 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4201 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4261 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4321 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4381 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4441 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     4501 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4561 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4621 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4741 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4801 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4861 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4921 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4981 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5041 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5101 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5161 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5221 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5281 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5341 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5401 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5461 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5521 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5581 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5641 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5701 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5761 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     5821 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
